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2016-02-26 ҵѧ

ֳPRRSVƶײԱŷޣ ֮ѸֹҺ͵Ŀԭԣ 죬PRRSVɷΪ2,ŷͣLVΪ꣩ͣATCC-VR2332Ϊ꣩PRRSV Ⱦȡʳ,ĸ̥̥ ľ ̥,(ر)ϰΪĸȾһ ֻݡ԰ף ֢ȾPRRSVȺƣ̷ϸԼ

 

2006꣬ ָ² 䴫Ѹ٣ Ⱥʧ Ⱥ  ֢״գ4042 棩ƤʳϾ׼̷Ⱦ NSP230  ȱʧ ҲΪ ͨ  ־

 

2030ShopeȱƣϸϸȾصϵͳ[5] ںϵͳۺԼáȾ ϸԭ ȾĤ ߸֧Ѫʿ˾Ѫ˾֧ԭסϸ˾ɳϾȣPRRSVȾ̷Ⱥӣ  һ ϸȾЭͬǿã ߣ ˡ

 

 ij1½15ͷ  IJЧ һԹͬвο

 

 

20157ԥij1½3ѱֻߡ֢״԰ס䷢ϣͼ1ͻȻ ͼ2ݵԡ ик֢״ Աƣ1ܺЧѳͬ ͬҲʽϸߡ

 

ͼ1   ֻ

 

ͼ1   ֻ

 

ͼ2   ֻͻȻ

 

ͼ2   ֻͻȻ

 

2֢״

 

  ڶǰã2ܺ ԡݲ԰ףߣͬ ڽͻȻ ط֢״ͼ31ֻܺ ͻȻЩϳɫƣͼ4

 

ͼ3   ֢״

 

ͼ3    ֢״

 

ͼ4   ϵϺɫ

 

ͼ4   Ϻɫ 

 

3ʼ

 

ںд ĭͼ5İĤ л ɫ Һͼ6ػ Ѫͼ7״Ƣ๣ͼ8 ɫ л㣬ܰͽ״ Ѫһϵ  䡣

 

ͼ5   дĭ

 

ͼ5   д ĭ

 

ͼ6   	ĤɫҺ

 

ͼ6   Ĥɫ Һ

 

ͼ7

 

ͼ7  

 

ͼ8   Ƣ	

 

ͼ8   Ƣ   

 

4

 

4.1 ԭ

 

ȡķࡢࡢƢࡢ֯塢 ܰ֯ʵ  αȮԭPCR⣬ԭԣСΪ370 bp Ϊ Ⱦͼ9

 

ͼ9   ֯ƷPCR

 

ͼ9   ֯ƷPCR

 

4.2 ϸ

 

ɼĸΡƢѪأˮؽҺȣ Ѫ֬ƽ 37 棬5%CO224 hȾɫ΢۲죬Ϊ  ԣͼ10

 

ͼ10    ϸ 

 

ͼ10    ϸ 

 

Ⱥٴ֢״ʼ  Ϊ ̷ 

 

 

5.1 Ⱥ ʩ

 

Ⱥñ  ǰʹ׿ҩ710 dҲ ͷҩάϼ ҩԤ

 

5.2 ѡ 

 

ѡȫȶͬԴԸߡȺ ƥ  ֣ ĸ ߣͨĸԴĸ1νֻ  ȱʧ ²磬4ټǿ1ΣԺÿ4Σ2 mL/ͷ̥141Σ ǰ4ܽ⣬34ټǿ1Σÿ2 mL/ͷ ֢״ֵֻ  Ⱥ  ע ؼ ơ

 

5.3 ǿȺʩ 

 

Ⱥ ǿעù ȾֵȣӲβš׶  ֹȾ

 

6

 

 ĸȺ   磬ЧԶͣã û ֹκ 磬 ߲² Ⱦ  ֻ   ͬȺգ ࣬4050̷   ֻPRRSV Ⱦ 57[9] Է ϸԣоԱPRRSVķݾ ϸ  Ⱦؼ ء

 

  ճ ע ȫϵ衣 һ ϵͳ̣Աơֳϸڹ ֵ߽ȡ֮ أ ֹ ķϸԼ Ⱦҩѵʹã ɼ

 

ԭ⣺² Ⱦ ضԲ


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